Olivia McCubbin Stepanic

Understanding Enzymatic C-H Bond Activation: A Combined Spectroscopic and Theoretical Approac

  • Understanding enzymatic C-H bond activation reactions via X-ray absorption and emission spectroscopies of soluble monooxygenases and relevant model complexes

  • Gaining mechanistic and structural insight from enzymes will aid in development of solvent-free, low temperature (120-150 °C) and moderate pressure processes.

  • Using microfluidic mixers and simultaneous X-ray spectroscopy and UV/vis spectroscopy as a way to access protein intermediate states in situ


Cutsail, G. E.; Banerjee, R.; Rice, D. B.; McCubbin Stepanic, O.; Lipscomb, J. D.; DeBeer, S., Determination of the iron(IV) local spin states of the Q intermediate of soluble methane monooxygenase by Kβ X-ray emission spectroscopy. J. Biol. Inorg. Chem. 2022, 27, 573-582, https://doi.org/10.1007/s00775-022-01953-4.

Mathe, Z.; McCubbin Stepanic, O.; Peredkov, S.; DeBeer, S., Phosphorus Kβ X-ray emission spectroscopy detects non-covalent interactions of phosphate biomolecules in situ. Chem. Sci. 2021, 12, 7888-7901, https://doi.org/10.1039/D1SC01266E.

McCubbin Stepanic, O.; Ward, J.; Penner-Hahn, J. E.; Deb, A.; Bergmann, U.; DeBeer, S., Probing a Silent Metal: A Combined X-ray Absorption and Emission Spectroscopic Study of Biologically Relevant Zinc Complexes. Inorganic Chemistry 2020, 59, 13551-13560, https://doi.org/10.1021/acs.inorgchem.0c01931.

Olivia McCubbin Stepanic (PhD representative)

Group: Prof. Serena DeBeer, Department of Inorganic Spectroscopy, Max Planck Institute for Chemical Energy Conversion